Place the seal on the piston/screw.

Put 100 to 500 mg of tissue into the barrel of the disposable liner. It is usually not necessary to pre-chop the tissue.

Insert the piston/screw into the barrel of the liner and slide the assembly into the press cylinder.

Screw down the press. Extract exits from the pointed end of the liner. With very small samples, the extract may remain in the liner tip but can be removed with a micropipette or small spatula.

Continue screwing down the press until all of the sample has been extracted. More pressure beyond this point will shear the plastic tip and force it out of the press cylinder. However, should this happen the liner is a disposable item and no harm is done.

Unscrew the press and remove the used liner and the used piston seal. The white poker provided is sometimes helpful. Proceed to the next sample.
 

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Last modified: 09/21/07.