Designed for high-throughput analytical
screening, the new MiniBeadbeater-96™, efficiently and
safely disrupts up to 192 samples of spores, microorganisms, plant and animal
tissue or soil samples at a time . Using the same efficient homogenization
process as its predecessors, the MiniBeadbeater-1
and -8, the sample and a large number of tiny ceramic beads are violently shaken
for 2-3 minutes to achieve 90+ percent cell disruption. The MBB-96
platform holds one or two deep well microplates or up to 45 standard microvials. The plates,
vials and beads
are disposable, thus making the method ideal for PAGE, PCR and Probe
applications where cross contamination between samples cannot be tolerated.
Compatible with phenol, Gu-SCN or various commercial kit solutions, RNA/DNA is
quickly recovered in the highest possible yields by doing the cell disruption
and nucleic acid extraction simultaneously. See also Comparative Selection Guide “Choosing
a High-Throughput Cell Disrupter.”
Click here for Selected Literature Citations: DNA from soil, Disruption of spores, etc
Click here for Q&A's on the use of the Minibeadbeater
Click here for Suppliers of RNA/DNA extraction kits
Specifications:
- Single-phase induction motor, 1 hp, (11.2 amp @ 115VAC or 5.6 amp @ 230VAC), 18"H X 24"L X 16"W, 115 lbs net wt.
- Shaking Speed: 36 oscillations/second or 2100 rpm (speed reduced by 17% with 50Hz power source). Disruption efficiency comparable to that of lower capacity MBB models, thus protocols are interchangable.
- Shaking pattern: , Shaking top to bottom of vial or plate in a compressed figure-8 pattern, with vials held in a horizontal orientation. Throw: 1.25 in. (3.2 cm).
- Timer: 0 - 5 minutes with automatic reset.
Ordering Information
Cat. No. 1001, MiniBeadbeater-96. Holds one-2ml deep-well microplate or two-1ml deep well microplates or one vial rack containing up to 45 microvials). One vial rack (see part # 702VH45, below) is included with the MBB-96, $3700.00
Cat. No. 1001EUR, as above in 220-240 Volt A/C, 50 Hz, $3750.00
Accessories
Cat. No. 702L,
96-well manual microplate bead loader. Loads beads into all 96 wells of a
2 ml deep well microplate at one time. How it works: Load a 1 ml
deep well plate with beads, place a frame around the loaded plate, then insert
into the frame, upside down, a 2 ml deep well receiving plate. Transfer
the beads by inverting the assembly. Suitable for 0.1 to 6.35 mm diameter
beads and for all densities of bead including steel, $96.00
►
Cat
No. 104L, 96-well vacuum microplate bead loader. Much faster and
easier than Cat. No. 702L. How it works: Using a supplied compact vacuum
machine, beads are sucked up and held in a 96-place manifold having fine
stainless steel screens at the ends of the inlets. Each inlet holds 1 cc
of glass, zirconia or resin beads (0.1 to 1 mm dia). Steel beads are too heavy to vacuum up.
The trapped beads are placed over a receiving 2 ml deep well microplate and
transferred by switching off the vacuum machine. Specifications: 115 VAC, 6 Amps
(Euro 230V not available at this time), $228
Cat. No. 35, 1 1/2" rubber roller, for hand pressing mats on microplates.
Better than doing it with your fingers but for low volume work
only, $26.00
Cat. No. IT-EP-R,
Leverage-action mat press for easily and secure attachment of mats on microplates
of any size. Recommended for processing multiple numbers of microplates,
$1250.00 ►
Cat. No. 702VH45,
Vial rack with
a microplate footprint, black polypropylene, for all common 1.5 or 2.0 ml microvials,
vial capacity fourty-five. The flip side holds thirty-two 0.5 ml microvials.
One rack comes standard with a MBB-96. Extras racks available in packs of five, $27.00 per pack
Cat
No. 504VH12, Vial rack with a microplate footprint, capacity
twelve 7 ml screw-cap, polypropylene vials. For extraction and cell
disruption in sample volumes larger than can be accommodated in a 2 ml
microvial, $15
Cat. No. 702ALU,
vial rack, forty-eight position, black anodized aluminum, for 1.5 or 2.0ml microvials. Microplate
footprint. This solid metal rack can be pre-chilled in a refrigerator or freezer. Recommended for native protein or organelle isolation where cooling is
desirable during cell disruption. In general, cooling is not needed for nucleic
acid isolation. Cooled to liq. nitrogen temperatures, the holder
supports "dry" grinding of fresh tissue by the cryopulverization
technique,
$184.00 ea. - see also
BioPulverizer.
Deep Well MicroPlates and Mats
Cat. No. P-DW-20-C, Axygen brand 2.0ml deep-well polypropylene, 96 wells per plate, 50 plates/case, $300.00
Cat. No. P-DW-11-C, Axygen brand 1.1ml deep-well polypropylene, 96 wells per plate, 50 plates/case, $300.00
Cat. No. AM-ML-RD, Axygen brand sealing mats for above deepwell microplates, 50 mats/case, $250.00
