MiniBeadbeater-96
Designed for high-throughput analytical screening, the new MiniBeadbeater-96™, efficiently and safely disrupts up to 192 samples of spores, microorganisms, plant and animal tissue or soil samples at a time . Using the same efficient homogenization process of its predecessors, the MiniBeadbeater-1 and -8, the sample and a large number of tiny ceramic beads are violently shaken for 2-3 minutes to achieve 95+ percent cell disruption. The MBB-96 platform can hold 45 standard microvials in a vial holder (provided) or one or two standard 96 deep well microplates (order plates separately). The plates, vials and beads are disposable, thus making the method ideal for PAGE, PCR and Probe applications where cross contamination between samples cannot be tolerated. Compatible with phenol, Gu-SCN or various commercial kit solutions, RNA/DNA is quickly recovered in the highest possible yields by doing the cell disruption and nucleic acid extraction simultaneously. See also Comparative Selection Guide: “Choosing a Multi-sample Cell Disrupter.”
Click here for Selected Literature Citations: DNA from soil, Disruption of spores, etc
Click here for Q&A's on the use of the Minibeadbeater
Click here for Suppliers of RNA/DNA extraction kits
Specifications:
● HIGH ENERGY induction motor, 1 hp, (12 amp @ 115Volts A/C or 5.6 amp @ 230 Volts A/C), 18"H X 24"L X 16"W, 115 lbs net wt.
● Shaking Speed: 36 oscillations/second or 2100 oscillations/min. Disruption efficiency comparable to that of the three lower capacity MBB models, thus protocols are interchangable.
● Shaking pattern: Shaking top to bottom of vial or plate in a compressed figure-8 pattern, with vials held in a horizontal orientation. Throw: 1.25 in. (3.2 cm).
● Timer: 0 - 5 minutes with automatic reset.
● Sample Capacity: Holds up to 45 microvials (0.5, 1.5 & 2.0 ml), or one 2 ml deep-well microplate or two 1.1 ml deep-well microplates with each vial or well containing up to 400 mg (wet weight) of biomaterial.
● Two year full Warranty.
Ordering Information:
Note: The MBB-96 uses beads and commonly available 2 ml screw-cap microvials or Microplates. Please order beads and microvials separately. Go to the links beads and microvials for prices and guidance in selecting the correct size and type of beads and vials. Microplates are listed below. For Protocols for this machine, click here.
Cat. No. 1001, MiniBeadbeater-96, USA/Canada/Japan. 115 Volt A/C, 60 Hz. The MBB-96 requires a 20 amp dedicated circuit - i.e., no other power-hungry equipment plugged into that line. One microvial rack (see part # 702VH45, below) is included with the MBB-96. Please order beads, microvials, microplates and mats separately, $4400.
Cat. No. 1001EUR, as above in European 230 Volt A/C, 50 Hz, $4500. [NOTE for European buyers! The MBB-96 is not CE certified at this time (Jun 2007). Certification is in progress.]
Accessories
Cat. No. 702L,
96-well manual microplate bead loader. Loads 1 ml of beads into all 96 wells of a
2 ml deep well microplate at one time. How it works: Load a 1 ml
deep well plate with beads, place a frame around the loaded plate, then insert
into the frame, upside down, a 2 ml deep well receiving plate. Transfer
the beads by inverting the assembly. Suitable for 0.1 to 2.3 mm diameter
beads and for all bead densities including those make of steel. $107.
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For rapid loading of one or two 6.35 mm diameter glass or steel beads into a deep well microplate - often used with dried plant material - see Beadloader for a "make-it-yourself" loader.
Cat
No. 104L, 96-well vacuum microplate bead loader. Much faster and
easier than Cat. No. 702L. How it works: Using a supplied compact vacuum
machine, beads are sucked up and held in a 96-place manifold having fine
stainless steel screens at the ends of the inlets. Each inlet holds 1 cc
of glass, zirconia or resin beads (0.1 to 1 mm dia). Steel beads are too heavy to vacuum up.
The trapped beads are placed over a receiving 2 ml deep well microplate and
transferred by switching off the vacuum machine. Specifications: 115 VAC, 6 Amps
(Euro 230V not available), $248.
Cat. No. 35, 1 1/2" rubber roller, for hand pressing mats on microplates.
Better than doing it with your fingers but limited
to low volume applications, $29.
Cat. No. IT-EP-R, Leverage-action mat press for easily and secure attachment of mats on microplates of any size. Highly recommended for processing multiple numbers of microplates. Works with any size capacity of microplate. $1375. --►
Cat. No. 702VH45,
Vial rack with
a microplate footprint, black polypropylene plastic, for all common 1.5 or 2.0 ml microvials,
vial capacity forty-five. The flip side holds thirty-two 0.5 ml microvials.
One rack comes standard with a MBB-96. Extras racks sold in packs of five. $30 per
five pack.
Cat.
No. 504VH12, Vial rack with a microplate footprint, capacity
twelve 7 ml screw-cap, clear polycarbonate plastic. For polypropylene vials
Cat No. 3205. Used for extraction and cell
disruption of larger sample volumes which cannot be accommodated in a 2 ml
microvials, $15 each.
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Cat. No. 3205, 7 ml polypropylene vials with screw caps for the 504VH12 Vial Rack above. $92/case of 1000.
Cat. No. 702ALU,
vial rack, forty-eight position, black anodized, solid aluminum, for 1.5 or 2.0ml microvials. Microplate
footprint. This solid metal rack can be pre-chilled in a refrigerator or freezer.
Recommended for native protein or organelle isolation where cooling is
desirable during cell disruption. In general, cooling is not needed for nucleic
acid isolation. In another application, "dry" grinding of fresh tissue by the cryopulverization
technique (see also
BioPulverizer) is possible with the sample and
702ALU vial holder chilled to liquid nitrogen temperature, $188 each.
Deep Well MicroPlates and Mats
Cat. No. P-DW-20-C, Axygen brand 2.0ml deep-well polypropylene, 96 wells per plate, 50 plates/case, $330.
Cat. No. P-DW-11-C, Axygen brand 1.1ml deep-well polypropylene, 96 wells per plate, 50 plates/case, $330.
Cat. No. AM-ML-RD, Axygen brand sealing mats for above deepwell microplates, 50 mats/case, $275.
