Screw out the piston of the TissuePress to make room for the tissue. Remove the brown "cap" and the 500 micron stainless steel wire screen.  If the tissue is partially fibrous or covered with membrane, trim out the tough parts and/or precut the sample into small pieces.  Generally, the 2.0 sieve size that is molded into the cap is used for most applications. If you select the 500 micron stainless steel wire screen, center it on top of the clear TissuePress cylinder. Screw the brown "cap" on the clear cylinder section. Open the brown cover of the "cap"  (Note: the white part of the cap is a scrapping tool - it does not lift).  Crank down the piston to extrude the tissue. Quit when it becomes difficult to turn the piston. Do not attempt to use tools to extrude the tissue. It will break the TissuePress. Clean the TissuePress promptly after use. Unscrew the piston from the cylinder and unscrew the collar holding the sieve piece. Remove tissue debris and clean all parts with a non-abrasive detergent solution. Rinse, dry and reassemble.
 

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Last modified: 09/21/07.