Selected Applications Using the Mini-Beadbeater
Extraction from Soil & Wastes
Evaluation and Optimiztion of DNA Extraction and Purification Procedures for Soil and Sediment Samples, D.N. Miller et. al., Appl. Environ. Microbiology, Vol.65, p.4715-4724 (1999). Click for web copy
Rapid Method for Coextraction of DNA and RNA from Natural Environments for Analysis of Ribosomal DNA- and rRNA-based Microbial Community Composition, R.I. Griffiths et. al., Appl. Environ. Microbiology, Vol 66, p.5488-5491(2000)
Simultaneous DNA/RNA Extraction from Soil, a protocol based on a modified method of Griffiths et. al.(2000), from Eoin Brodie, April 2003. References a Fast-Prep machine. Call for MBB equivalent speed settings.
Improved Extraction of PCR-quality Community DNA from Digesta and Fecal Samples, Zhongtang Yu and Mark Morrison, BioTechniques, Vol 36, p.808-813 (2004). Content: Beadbeating gives "1.5 to 6 fold increase in DNA yield compared to other methods"
Comparitive analysis of fecal DNA extraction methods with phylogenetic microarray: Effective recovery of bacterial and archaeal DNA using mechnical cell lysis, Anne Solonen et al, J Microbiological Methods, Vol 81, p.127-134 (2010). Content: Four widely used methods are compared. DNA yields varied up to 35 fold. A modified protocol of Yu and Morrison was the best.
Virus & Spores
A Field Investigation of Bacillus anthracis Contamination of USDA and Other Washington, DC Buildings during the Antrax Attack of October 2001, James A. Higgins, et. al., Appl. Environ. Microbiology, Vol.69, p.593-599 (2003)
Pathogens in Plants