Stainless Steel Microvials
Stainless steel microvials have the same dimensions as standard microvials. They are used when grinding forces are too severe for plastic vials or when far superior heat transfer and control is desired. The microvials are resistant to attack from most reagents and are reusable.
Product Description
Cat. No. 2007, 1.8 ml stainless steel microvials. Polyethylene flange caps are included*. Package size: 100 in polybag.
* Tough silicone rubber caps are available separately. They are required when beadbeating dry or hard frozen samples using steel beads.
Features
- Same dimensions as standard, 2 ml screw-cap polypropylene microvials
- Stainless steel microvials stand up to dry milling using our high-energy Mini-Beadbeaters. Plastic vials can break.
- 10X better heat transfer for controlled cooling or heating of vial contents. Achieve faster temperature cycles when using PCR methods
- Designed by and manufactured by BioSpec Products
Other Information
Protocol for dry grinding small frozen samples at liquid nitrogen temperature (i.e., cryo-grinding): Three 3.2 mm dia. or two 6.25 mm dia. chrome-steel beads are added to either a 2 ml stainless steel vial. The vial and beads are brought to liq N2 temperature...without entrapping any liq N2 inside the vial. Then add hard frozen cartilage, skin or other tough tissue (pre-chopping the sample is not be necessary), quickly seal the vial with a non-frozen silicone rubber cap and grind for 5-15 seconds in a MiniBeadbeater. The sample will be powdered.
If extraction of intracellular biomolecules is to follow, promptly add liquid media of your choice to the still frozen, powdered sample in the original vial and beadbeat for 0.5-3 minutes. The optimal beadbeating time will vary with sample type and is empirically determined with a time study of 0.5, 1, 2, and 3 minutes of beadbeating.