The Mini-Beadbeater-24 disrupts 4 to 24 microbial or tissue samples with better than 95 percent efficiency. Cells are disrupted quickly and safely in the sealed system. The apparatus is easy to clean, has a small footprint and is essentially maintenance free. The machine offers advanced electronics for enhanced motor function and variable shaking speeds of 2000-3450 strokes/min.
Cat. No. 112011, Mini-BeadBeater-24, 115 volt, with safety shield and 2 ml vial cassette.
Cat. No. 112011EUR, Mini-Beadbeater-24, 230 volts, with safety shield and 2 ml cassette.
NOTE for European buyers! The Mini-Beadbeater-24 is CE certified.
The Mini-BeadBeater-24 disrupts microbial cells and plant and animal tissue by violently agitating four to twentyfour 2 ml screw-cap microvials containing small glass, ceramic or steel beads and 0.1 to 1 ml disruption buffer. Even resistant samples like yeast, spores or fibrous tissue are completely homogenized in 1-3 minutes. The non-foaming, aerosol-free method preserves enzymes and organelles. In the presence of nucleic acid extraction media such as phenol, Gu-SCN or a commercial kit solution, DNA or RNA is recovered in the highest possible yield. The method is ideal for PAGE, PCR applications, and diagnostics using antibody or oligonucleotide probes. Because the beads and vials are disposable, there is absolutely zero cross-contamination between samples - essential for PCR techniques.
Protocols developed using a different model of the Mini-BeadBeater are transferable with minimal modification. The performance of the Mini-BeadBeater equals or exceeds that of any other type or brand of cell disrupter.
The Mini-Beadbeater-24 can also be used for dry grinding. Here, steel beads are added to hard samples such as hair, bone, teeth, seeds and minerals and are completely powdered in 10-60 seconds of operation. Softer materials such as biological tissue, rubber or plastics can be powdered by pre-freezing the sample to liquid nitrogen temperatures (cryo-grinding). Dry gringing requires using special microvials resistant to breakage. When dry grinding with ceramic or steel beads at room temperature our 'XXTuff' microvials are recommended. Our stainless steel microvials are available for dry grinding with steel beads at cryo-temperatures.
- Power: 115 volts, 60 Hz, 7.5 amps or 230 volts, 50 Hz, 3.7 amps
- 10' D X 18' W X 12' H, 50 lbs
- Shaking pattern: Uses proven, more efficient near horizontal vial orientation
- Capacity: four to twenty four screw-cap microvials (0.5, 1.5, and 2.0 ml) each containing to 400 mg (wet weight) bio-sample
- Shaking speed: Continuously variable (analog slider), 2000-3450 strokes/min
- Throw or stroke displacement: 0.75 inches
- Timer: 0-5 minute digital, with auto reset
- Removable vial-holding cassette
- No imposed motor cool-down-time between each sample run
- The Mini-BeadBeater-24 uses standard screw-cap plastic microvials. Stainless steel microvials or special reinforced polypropylene microvials ( XXTuff vials ) are available for dry- or cryo-milling with steel beads. Eight larger capacity, 7 ml vials can be processed using an accessory vial-holding ring (see Parts and Accessories below) .
SHOPPING GUIDELINES FROM THE BIOSPEC 'TECH GUYS': BioSpec Products was the first to introduce 'beadbeating' cell disruption to the scientific laboratory 30 years ago. This method of cell disruption for small samples has replaced most traditional methods. In addition to the Company's current five models of beadbeater cell disrupter (and there is one more debuting early next year), a dozen other manufacturers offer similar microvial-shaking 'beadbeater' cell disrupters*. Most of them are well designed and fulfill the following guidelines for maximum cell disruption performance: the machines should have a shaking speed of at least 2000 rpm, a throw (or displacement) of the vial of at least 3/4 inches and a shaking pattern that maximizes bead circulation to a top to bottom direction within the vial. Some manufacturers use a numerical setting expressed in units of meters/second. This term presumes to represent shaking efficiency, but in practice is simply an adjustment of the speed of shaking.
Cell disruption performance settings in m/sec is not a universal, comprehensive measurement of energy of shaking or efficiency of grinding. It hinders comparisons between experimental cell disruption protocols with other laboratories using different bead mill cell disrupters. In contrast, a successful example of the synthesis of mechanical features into a single, useful performance term is g-force of a centrifuge. The g-force term permits scientists to reproduce centrifuge performance, no matter who designed and manufactured it.
Unfortunately, there is no comparable performance term for shaking cell disrupters. Factors influencing cell disruption performance are numerous and complex and cannot be expressed in a simple mathematical or physical formula. It is suggested that the following simplistic guide-line be followed when when using bead mills designed for cell disruption: Operate the shaking cell disrupter machine at its maximum available speed setting. Specialized beadbeater applications requiring operation of a bead mill at lower speeds are rare. In other words, if the objective is to disrupt cells, crank up the rpm of the machine and get the job done. The time of beadbeating will depend on the nature of the biological sample and the type of bead mill shaker used. With current high performance machines, 2-3 minutes give close to 100% cell disruption. If you are doing PCR work with nucleic acids and can settle for less than 100% cell disruption, shorter periods of beadbeating will suffice. If native proteins or intracellular organelles are being recovered, temperature control will be essential. With most high energy beadbeaters the grinding process increases the homogenate temperature about 10º per minute of beadbeating. Temperature control is not as important for nucleic acid extraction in nucleic acid extraction media.
Besides shaking speed, vial throw distance, vial shaking pattern and orientation and time of shaking, three equally important variables which determine efficiency of cell disruption are bead size, bead composition and bead load in the vial. These three variables must be optimized by the user, not only for the 'beadbeater' machine in use, but for the type of sample being investigated. While commercially available vials prefilled with beads offer generic solutions to these last three variables, one can usually get superior results and, at the same time, save expense by assembling your own beads into vials. Usually only one kind and size of bead is needed. The MBB operating instructions** give straight forward guidelines on how to do that, as does the present web site. And, the tech guys are there to help, too.
* There is another group of commercially available bead mills cell disrupters that use vigorous vortex motion to move the beads and sample suspension within the vial. Vortex-type beadbeaters tend to be equally as efficient in disrupting cells but can take up to 10 time longer to achieve complete disruption. Some of the guidelines above apply only to shaking-type beadbeaters.
**Operating Instructions for the MiniBeadbeater-24: See http://www.biospec.com/product/465/mini_beadbeater/
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