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MicroMincer

MicroMincer

Cat. No. 2941, MicroMincer laboratory press complete with 10 pack of liner/seals
Cat. No. 294103, Ten pack of extra liner/seals

Price: $
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Product Description

The MicroMincer is a compact, hand-operated screw press which mascerates 0.3 grams or less of soft fresh plant or animal tissue (e.g. muscle, liver, brain, epicotyl, pulpy leaf tissue) without sample loss, heat generation, foaming or aerosol problems. The sample, without added buffer, is pushed through a 250 micron stainless-steel sieve under thousands of pounds force. The resultant extract can be deposited directly in microtubes. The stainless steel MicroMincer utilizes disposable polypropylene liners. Thus, multiple samples can be quickly processed without cleaning or risk of contamination between samples.
Applications:

  • Microanalysis of cellular constituents.
  • Eliminates re-concentration of sample extract.
  • Direct sample extraction for PAGE and agar gel analysis.
  • Preparation of single-cell suspensions from tissue*


*DNA double-stranded breaks in mouse kidney cells with age, Narendra P Singh et. al., Biogerontology 2, 261-270 (2001)

Disaggregation of tissue for primary culture or staining:  A common method to isolate single cells from tissue samples involves enzymatic digestion of the extracellular connective matrix.  The first step in the process is to increase access of added digestive enzymes to the tissue by chopping the tissue into pieces with a scissors or sharp blades.  In a significant improvement, much smaller tissue pieces can be obtained by maceration of tissue samples with the MicroMincer.  This greatly speeds up the rate of enzymatic disaggregation and maximizes the yiield of undamaged, viable cells.  As an important added benefit, the MicroMincer reduces the tissue to pieces so small that diffusion of nutrients and gasses are not limiting during the prolonged incubation with digestive enzymes. (An brief review of Enzymatic Disaggregation is found at http://www.worthington-biochem.com/tissuedissociation/default.html.)

How it Works:

  1. Place the seal on the piston/screw.
  2. Put 100 to 500 mg of tissue into the barrel of the disposable liner. It is usually not necessary to pre-chop the tissue.
  3. Insert the piston/screw into the barrel of the liner and slide the assembly into the press cylinder.
  4. Screw down the press. Mascerated tissue exits from the pointed end of the liner having a consistancy of toothpaste.
  5. Continue screwing down the press until all of the sample has been extracted (with very small samples, some of the extract may remain in the liner tip but can be easily removed with a small spatula). More pressure beyond this point will shear the plastic tip and force it out of the stainless steel press cylinder. However, should this happen, the liner is a disposable item and no harm is done.
  6. Unscrew the press and remove the used liner and the used piston seal. The metal poker provided is sometimes helpful. Proceed to the next sample.

Features

  • Directly mascerates small samples of soft tissue (e.g., liver, muscle, brain) - without dilution with added buffers or solvent.
  • Simple to operate - no need for compressed gases or high pressure electro-mechanical pumps.
  • No heat, foam, or aerosols formed.
  • The only parts in contact with the sample are the polypropylene cylinder liner containing a 250 micron stainless steel screen and the rubber piston seal (all disposable).
  • Develops thousands of pounds/square inch to mince the entire sample. No Product is lost.
  • Disposable parts eliminate cleanup and contamination between samples.

Specifications

  • Sample is pressed at a controlled speed through a screen having 250 micron openings.
  • Working capacity of 100 to 500 mg fresh, frozen or rehydrated plant or animal tissue.
  • 316 stainless steel body, piston/screw and screen.
  • Cylinder liner and piston seal made of polypropylene and ethylene-propylene rubber, respectively.
  • All parts autoclavable.
  • Size: 2 cm diameter, 11 cm high, weight 300g.

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