Tissue Extraction Tools for Medium Scale-up - A Selection Table
CLICK HERE for general tips on selecting mechanical homogenizers used for the extraction of plant and animal tissues at medium production levels. Select links below for tissue categories or specific homogenizers.
Hamilton Beach is the worlds largest manufacturer of commercial grade blenders. The model 909 comes with a one liter stainless steel container and can handle the toughest job required from a blade blender.
Cat. No. 909, 115 volt model
Cat. No. 909EUR, 230 volt model
The BeadBeater is the ultimate laboratory-scale homogenizer for disrupting aqueous suspensions of yeast, fungi or bacteria. Complete cell lysis of 10-80 g (wet weight) of cells is achieved in about three minutes of operation.
The BeadBeater comes complete with a 350 ml polycarbonate chamber, rotor assembly, motor base, ice-water cooling jacket, and your choice of one pound of glass beads...
Select the catalog number ending in...
-101 for 0.1 mm glass beads
-105 for 0.5 mm glass beads
-110 for 1.0 mm glass beads
Also, if the BeadBeater is to be used in countries with a 220-240 volt power supply, please select the catalog number containing "EUR."
The BioHomogenizer is a rotor-stator type tissue homogenizer designed to rapidly homogenize, disperse, and emulsify biological samples in aqueous media from 1 to 1000 ml. The BioHomogenizer includes both the motor and probe.
Cat. No. 1281, BioHomogenizer for 1-200ml samples, complete with 1.4 cm probe
Cat. No. 1281EUR, 230 volt version of No. 1281
Cat. No. 1285, BioHomogenizer for 100-2000ml samples, complete with 2.5 cm probe
Cat. No. 1285EUR, 230 volt version of No. 1285
Ideal for a one step preparation of cell lysate from most fruits and vegetables. Suitable tissues include tubers, squash, leafy tissues, fruits and pods. One of the few methods that do not require added liquid to obtain a cell free extract. Consequently, the highly concentrated proteins and biochemicals in the homogenate optimize down-stream purification or analysis steps. Not suitable for extracting animal tissue.
This classic tool has two applications in the molecular biology area: 1) Preparing large amounts tissue (100g - >1 kg) for subsequent fast and complete cell lysis using a beadbeater, ultrasonicator, rotor-stator machine or high shear press and 2) recovering large quanties of viable single cells following incubation of the dispersed tissue with proteolytic enzymes that digest extracellular matrix.
The electric flour mill is a high speed impact-type mill. Up to 2 liters of dry, non-oily seeds are quickly reduced to a fine flour-like powder. Subsequent solvent extraction of the high-surface area powder gently releases enzymes, proteins and other intracellular biochemicals. Examples of suitable seeds and grains are wheat, rye, rice, millet, corn, barley, dried beans and peas. Importantly, moisture content and oil content of the seeds should not exceed 15%. Examples of unsuitable seeds are sunflower, nuts, sesame, coffee, and peanuts.
A modified, top-of-the-line domestic waste disposer that sits on top of the lab benchready ready to plug in . Closed loop plumbing has been added to the basic unit to permit recycling the contents. The Semi-Continous Tissue Disperser delivers a high quality homogenate of almost any plant or animal material. Kilogram quantities of material can be processed in minutes in either a batch- or a semi-continuous mode. Operates at room temperature or in a cold room.