BioSqueezer Cryogenic Tissue Clamp

The BioSqueezer is used to quickly freeze animal and human tissue samples.  Tissue is compressed into a thin disc under a metal clamp head that is pre-cooled in liquid nitrogen or dry ice.  Freezing occurs in a few milliseconds, thus giving a snapshot of metabolic events in the cells.  The hard frozen disc is easily fragmented or ground without thawing for subsequent extraction of labile nucleic acids, proteins and intracellular metabolite products. 

Operation of the BioSqeezer:  A small beaker, styrofoam cup or similiar vessel is placed on the platform and the metal clamp is lowered.  Liquid N2 is slowly and carefully poured into the vessel until the boiling action of the liqN2 subsides.  This cooling process takes about a minute and, once cooled to the desired temperature, the stainless or aluminum metal clamp head will remain adequately cold for several minutes.  The vessel is removed and the sample, by itself or already in a poly-bag, is placed on the platform and the knob quickly lowered and clamped.  The clamping step must be done rapidly or the sample will freeze uncompressed.
It may be necessary to adjust the BioSqeezer using 'practice' tissue.  The optimal clamp setting can vary with the softness and size of the tissue sample.  Clamping force and the degree of squeezing on the tissue can be adjusted by changing the position of the metal clamp head with the wing nut, black plastic nut and the stainless steel hex bolt.
The thin waffer of tissue can be manually fragmented inside the poly bag while still frozen.  Presenting the tissue in small, thin pieces is ideal for quick homogenization and extraction or for storage in appropriate stabilizing solutions such as RNAlater or other excipients.