Stainless Steel Microvials have the same outer dimensions and weight as standard 2 ml microvials. They can be sealed with polyethylene flange caps, silicone rubber stopper caps or crimped septa caps.
- Dry grinding in a Bead mill (MiniBeadbeater) where milling forces are too severe for common plastic microvials2.
- Achieving far superior heat transfer - as might be required for PCR methods.
- Superior temperature control of samples in microvials.
- Milling or beadbeating of non-biological samples where trace contamination with polypropylene particles abraded from a microvial must be avoided.
1. NOTE! Special Silicone Rubber Caps are required when beadbeating dry or hard frozen samples using steel beads in the Stainless Steel Microvials. Scroll down for information on Silicone Rubber Caps.
2. An important exception is our new XXTuff polypropylene microvial. It is the the toughest polypropylene vial available for dry milling applications.
- Same physical dimensions as standard, 2 ml screw-cap polypropylene microvials
- Stainless steel microvials stand up well to dry milling (i.e., no liquid present in the vial) in high-energy cell disrupters such as our Mini-Beadbeater. Ordinary plastic microvials can break during beadbeating - especially when using steel beads as a bead media. (One exception is our recently introduced XXTuff™ polypropylene reinforced microvial. See http://www.biospec.com/product/462/xxtuff_reinforced_microvials/ for details.)
- Huge improvement in temperature control and heat transfer. The thin wall, stainless steel body of our microvial gives 10X better heat transfer than plastic microvials. This makes possible much faster temperature cycles when using PCR thermo cyclers and having shorter cooling intervals in an ice/water bath when following a protocol that requires periodic cooling during cell disruption.
- Designed by and exclusively manufactured for BioSpec Products.
A protocol for dry grinding small frozen samples at liquid nitrogen temperature (i.e., cryo-grinding): Three 3.2 mm dia. chrome-steel beads are added to a 2 ml stainless steel vial. The vial and beads are brought to liquid nitrogen temperature...without entrapping any liquid nitrogen inside the vial. Then add hard frozen cartilage, skin or other tough tissue (pre-chopping the sample is often not necessary)*, quickly seal the vial with a non-frozen silicone rubber cap and grind for 5-15 seconds in a MiniBeadbeater. The sample will be powdered.
If extraction of intracellular biomolecules is to follow, promptly add liquid media of your choice to the still frozen, powdered sample in the original vial and beadbeat for 0.5-3 minutes. The optimal beadbeating time will vary with sample type and should be empirically determined with a time study of 0.5, 1, 2, and 3 minutes of beadbeating.
* Use pre-frozen tissue. Fresh tissue will be "stuck" to the cold steel beads and delay good grinding