Stainless Steel Microvials - 2 ml

 

Cat. No. 2007, 1.8 ml capacity type 305 stainless steel microvials.   Polyethylene flange caps are included1.  Package size: 100 pieces

Our Price : $0.00

Our Stainless Steel Microvials have the same physical dimensions and weight as standard 2 ml polypropylene microvials. They can be sealed with either polyethylene flange caps, silicone rubber stopper caps1 or crimped 13 mm OD septa caps.

Applications include:

  • Dry grinding with steel beads in a high energy Bead mill cell disrupter (e.g.,MiniBeadbeater) where milling forces can crack common plastic microvials - - an exception is our 2 ml XXTuff polypropylene microvials.   They are the toughest 2 ml screw-top polypropylene vial on the market and can be used for most dry milling applications).
  • Provides far superior heat transfer capabilities compared to plastic or glass vessels - as might be desired for some PCR methods and the control of sample heating during the beadbeating  process.


 1  NOTE:  When beadbeating dry or hard frozen samples using steel beads Silicone Rubber Caps, specially shaped to seal our s.s. microvials, should be used. Flange caps can fail under these milling conditions.  Scroll down for more information on Silicone Rubber Caps.

 

A protocol for dry grinding small frozen samples at liquid nitrogen temperature (i.e., cryo-grinding): Three 3.2 mm dia. chrome-steel beads are added to a 2 ml stainless steel vial. The vial and beads are brought to liquid nitrogen temperature...without entrapping any liquid nitrogen inside the vial. Then add hard frozen cartilage, skin or other tough tissue (pre-chopping the sample is usually not necessary)*, quickly seal the vial with a non-frozen silicone rubber cap and grind for 5-15 seconds in a MiniBeadbeater. The sample will be powdered.
If extraction of intracellular biomolecules is to follow, promptly add ice-cold liquid media of your choice to the still frozen, powdered sample in the original vial and beadbeat for up to 3 minutes. The optimal beadbeating time will vary with sample type and should be empirically determined with an initial time study of 0.5, 1, 2, and 3 minutes of beadbeating.


   *  Add pre-frozen tissue.  Fresh tissue will "stick" to the cold steel beads and delay good grinding.