The BioPulverizer quickly fragments hard frozen tissue samples into tiny pieces the size of grains of sand or coarse powder. The primary benefit of pulverizing pieces of tissue is to optimize subsequent rapid and complete cell lysis using lytic solutions or mechanical homogenizers. The BioPulverizer can also be used at room temperature to fragment friable, dry biomaterial such as non-oily seeds, bone, and teeth.
There are four different hand-operated BioPulverizers with well sizes optimized for quickly pulverizing 10 mg to 10 g of frozen tissue.
Cat. No. 59012N, BioPulverizer, capacity 10-100mg
Cat. No. 59013N, BioPulverizer, capacity 0.1-1g
Cat. No. 59014N, BioPulverizer, capacity 1-10g
Cat. No. 59012MS, MultiSample BioPulverizer, 12 wells, capacity 10-100mg per well*
*Twelve pestles are included with each 59012MS.
Because of the high water content, most fresh tissue becomes as brittle as glass at liquid nitrogen temperatures. BioSpec Product's BioPulverizer reduces tissue samples hard-frozen in liquid nitrogen to a powder. A sharp blow to the tissue with an included hammer will shatter it into tiny pieces. Called freeze-fracturing or cryopulverization, the method is especially useful for preparing tough, fibrous tissues such as skin, cartilage, cornea, etc. for subsequent rapid cell disruption using other homogenizing techniques at room temperature.
The Bio-Pulverizer consists of a hole machined in a stainless steel base that fits a special stainless steel piston, or pestle. In a typical procedure, up to 10 g of animal or plant tissue is hard-frozen in liquid nitrogen and placed in the well of the liquid nitrogen-chilled BioPulverizer. Next, the pre-chilled piston is placed on top of the tissue in the well and struck by a hammer. After 2 or 3 blows, the brittle tissue is reduced to a powder.
Cryopulverization with the BioPulverizer is also useful for extracting labile tissue metabolites. And, for affinity proteomics studies, cryogrinding tissue in a MiniBeadbeater is a preferred way to disrupt cells for recovery of native protein complexes (see Biotechniques 58:103-119 (Mar 2015)).
How The BioPulverizer MS Works...
The 59012 MS Multi-Well BioPulverizer is made of stainless steel. The mass of the stainless steel will retain cryo-temperatures long enough to complete pulverization without a need for additional liquid nitrogen. After being cooled the Multi-Well BioPulverizer to liquid N2 temperatures, samples of hard frozen tissue are added to each of the 12 wells. One by one, a stainless steel pestle is inserted into each well and given a few blows with a provided dead-blow hammer. After removing the pestle, the cylindrical stainless steel sleeves are lifted off the base or platform, thus making accessible the hard frozen, powdered tissue remaining in a shallow well of the base. Working quickly, the still-frozen powdered tissue is removed with a spatula and transferred to a user-provided tube for processing in a buffer or nucleic acid extraction media.
- Type 316 stainless steel.
- Four sizes available.
- Hammer provided.
- Requires access to dry ice or, preferably, about 200 ml of liquid nitrogen.
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