Screw Tissue Press
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CELL LYSIS The Screw Tissue Press is used to initially disperse soft animal tissue (e.g., muscle, kidney, spleen, liver) before using mechanical, detergent, or chemical-based methods to lyse the cells. Compared to manually chopping using scissors or a scalpel, much smaller tissue fragments are produced and the cell lysis that follows will be faster and more complete. Notably, the addition of buffer is not required to use the Screw Tissue Press. The dispersed tissue exits the Screw Tissue Press having a consistency much like liver paté or toothpaste.
SINGLE CELL PREPARATION The Screw Press is also used in the preparation of viable single cells from soft animal tissue by enzymatic digestion of connective matrix. Screw tissue Press dispersion delivers higher yields of viable cells by significantly reducing incubation time with proteases and enhancing the diffusion of essential nutrients and gases during the incubation with the enzymes.
Key Single-cell Preparation references:
A Rapid Method for the Preparation of Single‐cell Suspensions from Solid Tissues, N. P. Singh, Cytometry 31, 229–232, 1998, >>https://onlinelibrary.wiley.co...(SICI)1097-0320(19980301)31:3<229::AID-CYTO10>3.0.CO;2-T
1) Acceptible tissue is soft, relatively non-fibrous and has high water content. Examples are muscle, brain, liver, plant fruits, some plant roots and tubers. If the tissue is partially fibrous, fatty or covered with a tough membrane, first manually trim off the tough or fatty parts. Fibrous tissue will interfere with the passage of the soft tissue through the press and lower the yield of dispersed cell material. Skin, hide, connective tissue, plant stems, wood and dried tissues cannot be processed.
2) Partially retract the piston cylinder of the Screw Tissue Press to make room for the tissue. Unscrew the 'cap' with its array of holes from the bottom of the Screw Tissue Press and place the tissue sample into the cavity of the press.
Generally, the array of 2 mm diameter holes in the press cap is used. If your sample is soft, non-fibrous material with high water content, you can also use the included 500 micron stainless steel wire sieve. Carefully center the screen on the end of the press cylinder after adding the sample tissue and screw on the press cap.
3) Manually turn the piston to extrude the tissue. The dispersed tissue will exit the press having a paste- or granular-like texture. Depending on the amount of connective or fibrous tissue present in the sample, the yield of dispersed tissue will vary from 20-95%.
4) Extruded tissue material can be removed from the end of the Screw Tissue Press with a scalpel or single edge razor blade.
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