XXTuff Reinforced Microvials - 2 ml
Cat. No. 330TX821, XXTuff2™ non-sterile, reinforced 2 ml vials and caps, 200 count
New XXTuff2 Reinforced Vials
The new XXTuff2 microvial has the same dimensions as a standard 2 ml microcentrifuge vial with a screw cap and o-ring seal. However, it has been reinforced in critical areas to withstand the rigors of dry grinding using steel beads. Testing shows the XXTuff2 vial is over 10 times more durable than standard microvial.
Our new XXTuff2 vials improve on our earlier reinforced vial. The new XXTuff2 vials feature a skirted, free-standing bottom that reduces blow-outs to the bottom of the tube that can occur with some dry grinding protocols. The XXTuff2 vial also features a natural colored cap. This eliminates the red-sh tint some observed in their sample due to the abrasion of the red cap in our original XXTuff vials.
Vials are autoclavable and DNase and RNase free.
*When doing CryoGrinding, a variant of dry-grinding done at liq N2 temperatures, we recommend using our stainless steel microvials. All plastic microvials become too brittle to withstand beadbeating with steel beads at extremely low temperatures.
Other Info :
Protocol for dry grinding small frozen samples at liquid nitrogen temperature (i.e., cryo-grinding): Three 3.2 mm dia. or two 6.25 mm dia. chrome-steel beads are added to either a 2 ml stainless steel vial or an 'XXTuff' 2 ml polypropylene vial. The vial and beads are brought to liq N2 temperature...without entrapping any liq N2 inside the vial. Then add hard frozen cartilage, skin or other tough tissue (pre-chopping the sample is not be necessary), quickly seal the vial and grind for 10-15 seconds in a MiniBeadbeater. The sample will be powdered. Then promptly add liquid media of your choice to the still frozen, powdered sample in the original vial and beadbeat for 0.5-3 minutes. The optimal beadbeating time will vary with sample type and is empirically determined with a time study of 0.5, 1, 2, and 3 minutes of beadbeating.
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